The particle consists only of an extremely small circular RNA (ribonucleic acid) molecule, lacking the protein coat of a virus.
Nevertheless, as shown here, the expressed GFP-ATM1 which is a mutant molecule lacking the head, actin binding domain, did not disrupt the ER network.
For comparison, the unambiguous orientation of the estriol molecule lacking the aliphatic side chain in M. tuberculosis CYP51 (resolution 1.55 Å; PDB ID 1X8V) is consistent with the sterol orientation suggested by the T. brucei 3P99 structure.
The crystal structure of the bovine factor Va molecule lacking the entire A2 domain and the acidic COOH terminus of the heavy chain (factor Vai) has been determined (18).
The advantages of sCUR include that it is odorless and tasteless, more easily soluble in DMSO, and that it is a single, highly purified molecule, lacking the batch-to-batch variation of CUR content in nCUR.
In BAX-Δ, the in-frame fusion of exon 2 and exon 4 results in a molecule lacking the BH3 death domain predicted to be unable to dimerize with BCL-2 family members [ 24].
A mutant Xdsh molecule lacking the carboxy-terminus DEP domain but containing the DIX domain (Xdsh-dd2) was also able to block Xnr3 expression induced by ECD8 and Wnt-5a.
This mutant molecule, lacking the head, motor domain that bind actin but containing the neck and tail domains, is expected to bind to its cellular targets and function as dominant negative by blocking wt myosin binding.
We have recently demonstrated that a factor Va molecule lacking the last 30 amino acids from the carboxyl-terminal end of the heavy chain (amino acid residues 680−709) displayed a reduction in clotting activity and a delay in prothrombin consumption, leading to the accumulation of the intermediate meizothrombin during prothrombin activation.
Bearing in mind the poor fibrinogen clotting properties of meizothrombin, we can hypothesize that following vascular injury, individuals with a factor Va molecule lacking the COOH-terminal region of the heavy chain will produce more meizothrombin, rather than α-thrombin, at the place of vascular injury, resulting in mild bleeding tendencies.
