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TorsinA-bound ATP molecule is in red.
We hypothesized that the HLB of a dendron containing a small molecule ligand (complementary to a specific protein) would be significantly different from that of the protein dendron complex, because a small molecule is replaced by a rather large hydrophilic surface in the latter system (provided by the bound protein).
Direct evidence for the presence and composition of surface-bound linker molecules is provided by LDI mass spectrometry and by quantitative XPS analysis of trifluoroacetylated derivatives.
IR spectroscopy gives qualitative information about the way in which the adsorbed molecules are bounded to the surface as well as structural information of solids.
On the Illumina/Solexa platform, library molecules are captured by surface-tethered probes complementary to the adaptor sequences and are amplified by bridge PCR to convert a single DNA molecule into a surface-bound cluster with many copies of the same sequence.
Monomer molecules (THA) for surface-bound polymerization would be adsorbed at full coverage over TiO2 if the whole adsorption sites of TiO2 surface are occupied by polymer PTHA for polymer adsorption as the control.
SPR is particularly outstanding in biosensor applications, since SPR can measure the interaction of unlabeled molecules with surface-bound species in real time [47].
This is because the surface-bound probe molecules interact strongly with the surface, reducing the freedom of intramolecular rotation.
Also, it still remains to be determined how these numbers compare to C3b molecules on a natural biological surface where the thioester is anchored to surface-bound structures (polysaccharides, proteins) [ 9] that would allow more flexibility and enhance intermolecular contacts.
In particular, HLA-1a molecules are membrane-bound proteins, while HLA-1b molecules have membrane-bound isoforms and soluble isoforms [ 17].
A similar profile was observed for surface-bound TGF-β.
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