A molecule is labeled active if the corresponding EC50 is smaller than the selected cutoff, and inactive otherwise.
In PALM imaging, the target protein molecule is labeled with a photo-activatable fluorescent protein, such as EosFP [20].
The investigation of the association between streptavidin and biotinylated peptides requires that the fastest diffusing molecule is labeled with FITC while the slowest one is kept unlabeled.
Residues belonging to the symmetry related Hfq molecule are labelled with an asterisk in panel B. Side chains are shown for all residues, main chain is shown for G4 in both panels to help visualize the residue position.
Each molecule was labeled as either 1 or 2, and these labels were copied when molecules were replicated.
Our results compare well with those from a series of other single molecule studies where the molecule was labeled on the lever arm, the tail, or attached to a surface.
Residues belonging to the symmetry related Hfq molecules are labelled with an asterisk.
Singly biotinylated SSTR3 and Smo molecules were labelled on the cell surface by extracellular addition of a low concentration (50 pM) of monovalent streptavidin (mSA; Howarth et al., 2006) conjugated to Alexa647 fluorescent dye (mSA-A647).
Equal amounts of double-stranded rDSBs and pA70/pT70 or single-stranded pA70 and pT70 DNA molecules were labelled with P at the 5′ or 3′ ends and incubated in egg extracts.
