It seemed possible, however that not every pUC19 molecule was digested by HpaII before religation and amplification.
The ligated "YI" molecule was digested with the restriction endonuclease MmeI, a Type IIS restriction endonuclease that cuts 20 and 18 nt away from its recognition site, yielding a 2-nt 3' overhang.
These community and enzyme changes led to the conclusion that bacteria community differences do affect how large carbon molecules are digested.
The double-stranded (ds) cDNA molecules were digested by restriction enzyme SfiI and inserted into a pDNR-LIB vector and subsequently electrotransformed into E. coli DH10B.
The elongated and immobilized DNA molecules are digested with a restriction endonuclease of choice.
DNA elongation allows the imaging of molecular cleavage events once intact DNA molecules are digested using restriction endonucleases, and is an important requirement for generation of Rmaps.
In a comparative analysis undertaken previously [ 34], both supercoiled DNA molecules were digested with enzymes that altered their supercoiled DNA structure.
Fosmid molecules were digested with each enzyme either singly or in combination (the two enzymes generate compatible ends) to release a large internal fragment of the genomic insert while leaving behind ends of indeterminate length.
To determine whether encapsidated EBV rather than fragments of EBV DNA were present in culture supernatant of the SNK and SNT cell lines, 800 μl of supernatant of cell lines was treated with or without DNase I (Invitrogen, Carlsbad, CA, USA) by which cell-free EBV DNA molecules are digested (Gallagher et al, 1999).
