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By examining surfaces with low average RGD densities, our single molecule imaging experiments revealed that NIH-3T3 cells can tether to the substratum in areas of ~0.8 RGD peptides per µm2.
To structurally characterize the early molecular interactions of the protease with collagen type-II fragments we used AFM single molecule imaging experiments.
Fourth, due to a collective emission of abundant dopants within a single UCNP, luminescence from a single UCNP does not show the blinking behavior, which is important for single molecule imaging experiments involving a long-time observation.
Single molecule imaging experiments were performed in an epi-fluorescence microscope setup consisting of an inverted microscope (IX71, Olympus America Inc ., 1.45 NA TIRF objective (U-APO 150x NA 1.45, Olympus America Inc ., 635 nm diode laser (Radius 635, Coherent Inc ., and an electron multiplying CCD camera Ixon (897, Andor Technologies PLC). with EM gain set to ≈ 200.
In evaluating the success of performing single molecule imaging experiments on K562 suspension cells containing sensors, we found that the conditions were not ideal (Supporting Information Movie 1) even when restrained with poly- l-lysine, concanavalin A, DNA methods, or antibody conjugation to cell surface marker CD71 (data not shown).
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Furthermore, we present single-molecule imaging experiments that demonstrate that individual mKikGR proteins can be localized with a precision of better than 10 nanometers, suggesting their suitability for super-resolution imaging.
After fixing the labeling concentration for each cell line, we then proceeded to perform the 2D single-molecule imaging experiments.
In order to validate the model of revolution without the need for rotation, several single-molecule imaging experiments were carried out.
Single-molecule imaging experiments have shed new light on the methods used by the enzyme RecA to align single- and double-stranded DNA so that double-strand breaks can be repaired.
Second, recent single-molecule imaging experiments show that WASP dissociates from the branch junctions before elongation of the daughter filament (Martin et al., 2006) (Smith et al., 2013, in press at eLife).
The utility of these probes is underscored in single-molecule and super-resolution imaging experiments, which reveal Na(V) distributions well beyond the optical diffraction limit in subcellular features such as neuritic spines and filopodia.
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