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Sukhanova, M. V. et al. Single molecule detection of PARP1 and PARP2 interaction with DNA strand breaks and their poly(ADP-ribosyl)ation using high-resolution AFM imaging.
We demonstrated multicolour single molecule detection of signalling proteins on live cells using the human T47D breast cancer cell line.
The single molecule detection of molecules immobilised at a surface provides an extended observation window limited only by photobleaching, but requires care to avoid perturbation of biomolecules that may be induced by surface interactions.
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Single-molecule detection of the biomolecules in a label-free manner has a tremendous impact for various fields.
We review electrochemical biosensors based on dendrimer-encapsulated metal nanoparticles (NPs) and their various applications (e.g., measurement of small biological and toxic molecules, detection of DNA, and immunosensing of disease biomarkers).
In the past twenty years, SERS spectra have been successfully performed on investigating the interaction of the nanoparticles with the living cells, such as single-molecule detection of cellular proteins, the conformational transformation, and the structural change [8 11].
10.7554/eLife.01775.007 Figure 2. Real-time single-molecule detection of transcription by T7 RNAP using fastFISH.
10.7554/eLife.08363.005 Figure 2. Single-molecule detection of the TERT subunit content in DNA-bound complexes.
In this report, we have described an improved method for purely optical single-molecule detection of unlabeled analytes passing through solid-state nanopores.
In confocal experiments, single-molecule detection of free-flowing molecules is achieved through a combination of a small confocal probe volume (<1 fL) illuminated by focused laser beams and a low sample concentration, typically less than 100 pM.
We have successfully overcome this problem with a novel combination of two widely used approaches: target-specific multiplex amplification with 15 cycles of polymerase chain reaction (PCR), followed by single-molecule detection of amplicons with atomic force microscopy (AFM).
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