For the evaluation of the different film thickness on the quality of SERS spectra, R6G was also chosen as the probe molecule (detection method is the same as the previous work).
The center is also exploiting single-molecule detection methods to explore the mechanisms of basic biological interactions.
Our current research involves the study of a G-protein coupled receptor by single-molecule detection methods.
The Walker lab at Cornell University uses single-molecule detection methods, such as quantitative fluorescence microscopy, to study cellulase adsorption and hydrolysis [ 20].
At the same time, single-molecule detection methods such as Pacific Bioscience's PacBio RS and the recently announced Oxford Nanopore Technologies ONTT) GridION and MinION systems offer much longer read lengths, albeit at the expense of higher sequencing errors (PacBio RS: ∼10 15% and ONT GridION: ∼4%).
However, evidence is mounting from studies using specific antibody or small molecule-mediated detection methods to support their formation in vivo (Schaffitzel et al., 2001; Müller et al, 2010; Biffi et al., 2013; Lam et al., 2013; Henderson et al., 2014).
The single-molecule multicolour detection method is based on a simple principle: the two excitation lasers are focussed within the same point, creating a very small observation volume (∼1 fl).
Taking advantage of sensitive fluorescence detection methods, single-molecule imaging techniques have improved our understanding of the structure and function of proteins.
The Lee group at the Mara Nanotech has established this NW-based biosensing technology during the past two decades by engineering highly sensitive electrochemical sensors and providing a broad range of detection methods from large molecules (e.g., cells or proteins) to small molecules (e.g., DNA and RNA).
Our study has shown that protein microarray technology is a more sensitive, reliable and efficient method for small molecule detection than traditional ELISA.
Biologically driven detection and identification of a range of target molecules presents unique challenges, and requires that detection methods are developed to be rapid, specific and sensitive.
