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Pseudoterranova azarasi, the subject of the Etymologia, was originally described in 1942 as Porrocecum azarasi, but recent molecular work, as described by Arizono et al. (3 ) and Mattiucci and Nascetti (4 ), showed that this nematode is part of a large species complex within Pseudoterranova.
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MS collected samples, carried out the molecular systematic work as well as drafted the manuscript.
TM was responsible for the major part of the molecular sequencing work, as well as most analyses and the manuscript draft.
For the remaining amplifications we found 87% (220 of 261 LxH and 193 of 210 LxP) of the molecular markers to work as predicted.
While several formulations of HES plasma expander are commercially available, it is highly recommended to use high molecular weight HES 200/0.5 or 200/0.6 (mean molecular weight of 200 kDa), since formulations of lower molecular weight HES do not work as well as a sedimentation agents for getting leucocyte-rich plasma (LRP) from blood.
The fieldwork was conducted by AS, CC, GFT and MSt, and the molecular work (genotyping, mitotyping and sequencing) by AS, CC and MSt.
RMG carried out molecular work and contributed to experimental design as well as to the writing of the manuscript.
AMH carried out field work as well as molecular genetic studies.
The phylogenetic relationships within the clade are not yet fully resolved; a comprehensive morphological study [ 3] and ongoing molecular work [ 4- 6] serve as a framework in which to understand complex biogeographic and ecomorphological patterns of evolution.
Conversely, as molecular work helped to inform our models, our models also shed light on and confirm results of studies on the species' genetic structure, and possibly evolutionary trajectory.
AS carried out serology and molecular work.
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