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For a series of binary brushes consisting of PMMA of molecular weight of 26,200 and PS of various molecular weights, we have observed a transition in water contact angles with increasing PS molecular weight after CH2Cl2 treatment.
Based on their molecular weights, we speculate that the 33 kDa band is nucelophosmin, a known PAD substrate, and that the 10 and 15 kDa bands are likely histone H3 and H4.
Notably, their precursor proteins have a molecular weight of 136 to 311 kDa (according to SwissProt entries O25063, O25330 and O25579) which is in accordance with the molecular weights we observed in the zymography after a possible cleavage of the N-terminal signal peptide and the autotransporter sequence.
Knowing the number of molecules (Ghaemmaghami et al. 2003) and their molecular weights, we could calculate the total weight of every protein.
As the commercially available antibody reacts with the phosphorylated activation loops of all 3 PKDs that have similar molecular weights, we used GFP-PKD3 construct to distinguish it from the endogenous PKDs by size.
Taking into account the dynamic properties of all the proteins studied and their molecular weights, we asked whether the nuclear patterns influenced the kinetic properties of these proteins, or if molecular weight was the sole factor.
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By varying the molecular weight, we vary the thermodynamic product NχPVC/PS which controls the phase morphology.
Investigating these properties together with the complex modulus and the calculating cross-link molecular weight, we tried to determine how the network is constructed.
Since both dyes were comparable in molecular weight, we assumed a diffusion coefficient of 400 µm2/s for ATTO647N-maleimide as well.
Based on its molecular weight, we speculated that the protein around 45 kDa could be GSK-3β and an anti-P-GSK-3β specific antibody confirmed an increase in phosphorylation of GSK-3β.
To generate protein bands with a sharper aspect and a more defined molecular weight, we deglycosylated TMEM16A protein with PNGaseF.
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