The GPC column was standardized using polystyrene molecular weight standards in the range of 162 to 38,640 Da.
The amount of DNA used for DGGE was standardized to approximately 200 ng by comparison to molecular weight standards.
Lane 1 molecular weight standards.
The size of molecular weight standards is indicated on the left margin.
Vaccinated patient tested with anti-HBsAg isotype antibody 10, 12 molecular weight standards.
Molecular weight standards of polystyrene with a narrow polydispersity index were used for calibration.
Uncropped blots with molecular weight standards can be found in the Additional file 1: Figure S2.
More accurate molecular weight was deduced due to the use of PEGs as molecular weight standards.
Molecular weight standards from top to bottom are 94, 66.2, 45, 33, 26, 20 and 14.4 kDa.
The protein assay kit and SDS-PAGE molecular weight standards were obtained from Bio-Rad Laboratories (Hercules, CA, USA).
Molecular weight standards are run in the left two columns and a negative control, tissue from Mgam deficient adult CBA Ca strain mouse, in the right column.
