The basic properties of gases are commonly expressed in terms of the apparent molecular weight, standard volume, density, specific volume, and specific gravity.
Lane 1 Molecular weight standard, lane 2 purified β-xylosidase.
Lanes: M, molecular weight standard; 1, crude extract; 2, purified LrXynA.
MagicMark™ XP Western Protein Standard Thermo Fisher Scientific Inc.., MA, USA) was included as the molecular weight standard.
As references, a molecular weight standard protein collection Kaleidoscope marker (Bio-Rad, Munich, Germany) and untreated FCS were used.
A molecular weight standard (Bio-Rad Laboratories, HerCAles, CA) was loaded in the last lane of each gel to assess relative molecular mass of detected bands.
Lane 1, molecular weight standard; lane 2, lysate soluble fraction from E. coli induced with IPTG; lane 3, proteins that did not bind to Ni-NTA resin; lane 4, proteins eluted from Ni-NTA resin with wash buffer; lane 5, protein eluted from Ni-NTA resin with 300 mM imidazole; lane 6, pure CBM-SUMO eluted from size-exclusion chromatography.
PageRuler Plus (Fermentas) was used as molecular weight standard.
Each gel contained dual-prestained molecular weight standard (Bio Rad, Hercules, CA).
The molecular weight of extracted genomic DNA was c. 13 Kb (as determined by comparison to a molecular weight standard).
A protein molecular weight standard was run in parallel to estimate the approximate molecular weights of the proteins separated.
