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This was interpreted to be mono-ubiquitinylated STAT3 at 75 kDa and poly-ubiquitinylated STAT3 protein at the large molecular weight sizes.
Full length as well as many other molecular weight sizes, were detected by anti-His-tag antibody detection on Western Blot.
In contrast, the dog saliva pool revealed at least 12 IgE-binding proteins and several of those were of molecular weight sizes not recognised in the dog dander extract (Fig. 1).
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The migration of a molecular weight size marker is indicated.
The gel size was determined using standard molecular weight size markers after the bands were detected by silver staining.
By carefully planning the scheme of cores and branching units, the molecular weight, size, branch density, flexibility, and water solubility can be specified [49].
Based on the expected product size given in the GRAMENE website (Additional file 2: Table S1), the size of the most intensely amplified bands around the expected product size for each microsatellite marker was identified using standard molecular weight size markers (20 bp DNA ladder, GeNeI Company).
PCR products of the same molecular weight size as the PCR positive control were considered positive as long as the negative control was negative.
The T. pratense BAC library was constructed from partially digested gDNA in a single, high molecular weight, size selection experiment.
Because of the logarithmic scale in sizing fragments, small differences in migration of large fragments result in large differences in molecular weight size calculation.
The broad molecular weight size range measured was likely from the presence of 12-, 18-, 24- and 36-mers generally described – and isolated – as the HMW complexes.
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