Sentence examples for molecular weight shift from inspiring English sources

Glycosylation was verified by a molecular weight shift in SDS-PAGE after PNGase F (NEB, Ipswich, USA) treatment.

In fact, P301S and S305N isovariants within the basic pI range displayed a molecular weight shift.

More importantly, we observed that the molecular weight "shift" of mutated 4RTau isovariants is completely abolished after treatment by λ-phosphatase.

More importantly, 4RTau mutants isovariants showed a distinct 2D-migration pattern characterised by a molecular weight shift (towards L2 and L3), which is abolished after in vitro dephosphorylation.

Altogether, these data clearly support that, compared to WT 4RTau, mutated 4RTau likely display a difference in phosphorylation pattern which is responsible for a substantial molecular weight "shift".

We detected an intense band at 110kDa, which superimposes precisely with the HA-Isoform 2 band (the extra 10 amino-acids of the HA tag probably generate a molecular weight shift too small to be detected).

To prove that these molecular weight shifts were the consequence of glycosylation, cell lysates were treated with the endoglycosidase PNGaseF in order to cleave N-linked glycans.

Several of these proteins are known to undergo activation-induced post-translational modifications, including ubiquitination [10], potentially accounting for the molecular weight shifts.

Insertions at amino acids 13, 84, 177, and 254 did not result in molecular weight shifts corresponding to greater than 19 amino acids the molecular weight of the FNF tag (Fig. 1B).

Proteins of the vitellogenin family are widely distributed on the 2D gel and show pI as well as molecular weight shifts, which are due to modification through cleavage and to different PTMs like glycosylation and phosphorylation during development of oocytes.

Western blot analysis showed that FNF tags inserted at amino acid positions 51, 129 and 217 resulted in molecular weight shifts greater than the 19 amino acid addition of the FNF tag, likely indicating glycosylated FIT2-V5 (Fig. 1B).