As expected from its predicted and observed molecular weight, antibodies raised against recombinant TLP1 [ 1] detected a 23 kDa protein in poplar phloem exudate and in green stem tissue peeled from wood of poplar saplings, hereafter referred to as 'bark' (Fig. 2A).
First, in the absence of even these low doses of detergent, the elution profiles show almost exclusively higher molecular weight assemblies, raising the question of whether the reported molecular size corresponds to the neurotoxin itself or merely to a stable fragment [ 22, 23, 87].
The sculpture's weight raised concerns.
Additional O-linked glycosylation by heparan and chondroitin sulphate glycosaminoglycan chains, together with N-linked carbohydrates, raise the molecular weight up to 400 600 kDa [14], [15] (reviewed in [8]).
First, post-translational modifications such as phosphorylation may raise the molecular weight of the protein.
Using analytical gel filtration, column retention times of hFAN1 and mFAN1 were consistent with a molecular weight of approximately 200 kDa, raising the possibility of a dimeric interaction.
In homo-brush as well as single-co-crystals, through increasing the molecular weight of amorphous blocks, the total thickness raised while the crystalline substrate thickness decreased.
Electrophoretic mobility of HA nanoparticles was raised by decreasing the molecular weight of alcohol, so the deposition rate of methanolic suspensions was the highest.
A monoclonal antibody raised against the high molecular weight melanoma antigen was labelled with indium-111 and injected intravenously into 25 patients with malignant melanoma.
Following deglycosylation, RT4, SW780 and RT112 retained higher molecular weight forms (Supplementary Material, Fig. S1A), raising the possibility that these represent fusion proteins.
