However, initial analysis of JV polyprotein processing indicated that only two major proteins are synthesised initially which, based on molecular weight predictions, are the 3ABCD (110 kDa) and the 2C (39 kDa).
Molecular weight predictions and sequence identity and similarity analyses were performed with the Protein molecular weight function and ident and sim functions of the Sequence manipulation suite [ 58].
These sizes were agreed with the theoretically molecular weights prediction of the expressed rOMP25, rOMP28 and rOMP31 fusion proteins respectively.
The full-length cDNA sequences analysis and interpretation were carried out using the Bioedit software (Version 5.0.6) for determination of molecular weight and prediction of acid nucleus content.
For each protein, its Pfam annotation, Phyre2 structure prediction, molecular weight and signal peptide predicted by SignalP are given as well as the distribution of significant phmmer hits against the non-redundant protein databank (NR) in terms of taxonomy.
The first group consists of thermodynamic models, which need asphaltene properties such as density, molecular weight and solubility parameter for prediction of asphaltene phase behavior.
Unsupervised clustering of the pathogen-associated protein set according to 35 sequence-derived features (e.g. signal peptide prediction score, molecular weight, amino acid composition) leads to protein clusters which share similar characteristics.
To dissect the nature of the pathogen-associated proteins we next used an unsupervised clustering method based on 35 features (e.g. signal peptide prediction score, molecular weight, amino acid composition), deduced from the sequences.
Model predictions on molecular weight distributions are in agreement with experimental trends.
Therefore, prediction of lower molecular weight, reagent-like aryl-amines were the principal interest.
