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The appropriate choice of the flanking residues allowed us to design virtual lead compounds, such as FP14, FP23 and FP76, with reduced molecular weight, predicted inhibitory potencies in the picomolar range, promising ADME profiles and a potential to escape drug resistance due to favourable interactions with the PR backbone.
The absolute molecular weight, predicted to remain constant, changes only slightly and is consistent with a primarily unimolecular phenomenon (Table 2).
As expected, our antibody detected a protein band of the molecular weight predicted for A1 in ConA-stimulated GFP-negative cells but not in the GFP-positive (i.e. A1 shRNA expressing) splenocytes.
This suggested that the phytase protein was partially glycosylated in P. pastoris and the observed molecular weights were higher than the molecular weight predicted from the amino acid sequence alone (46.3 kDa).
The connexin nomenclature is according to one of two systems: (i) The first is based on a "number" system whereby the molecular weight predicted from the cDNA sequence of the connexins denotes the type of the connexin.
A single band with the molecular weight predicted for survivin was detectable by immunoblot analysis in whole cell lysates from proliferating mouse and guinea pig fibroblasts as well as from a human tumor, which served as the positive control (data not shown).
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The observed molecular masses of the majority of the identified proteins matched well to the theoretical molecular weights predicted from the amino acid sequences of the database entries (Table 1).
The electrophoretic mobility of these two isoforms as resolved by SDS-PAGE is in agreement with the molecular weights predicted from the respective amino acid compositions (EZH2α: 86.03 kDa; EZH2β: 81.05 kDa).
DU145 and MCF10A cells predominantly express two p120ctn isoforms that, based on their molecular weight, are predicted to be isoforms 1 and 3 [33] with isoform 3 (lower band) being expressed at slightly higher levels (Fig. 1).
Molecular weight and predicted band size of LDH is 37 kDa.
Molecular weight and predicted band size of AChE is 68 kDa.
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