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The bioavailability of PAHs generally decreased with increasing molecular weight (Figure 4), although the bioavailable proportions of Nap were smaller than those of 3- or 4-ring PAHs at certain stations, such as Stas.
The spectrum of SPGO-PEG silane2000 is very similar with that of SPGO-PEG silane550, and they have very little differences most likely due to size effects or molecular weight (Figure 3b, e). Figure 3 FTIR spectra for DEG PEGylated nanoparticles.
The sequence of the unknown protein can be optionally provided and is used to calculate the molecular weight (Figure 1a).
Immunoblot analysis confirmed that the FRET donor and acceptor proteins were all expressed and of the correct molecular weight (Figure 1A).
From the descriptors which quantify different aspects of molecular size, minimum projection area was found to show somewhat higher correlation than molecular weight (Figure 4N O).
STF2.HA1-1 (PR8) had a predominant band migrating at the correct size and a minor band migrating at a higher apparent molecular weight (Figure 2A).
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It is also clear that PAHs with low molecular weights had higher bioavailable percentages than those with high molecular weights (Figure 4).
Compared to a reference set of molecules with known subcellular localization features [31, 32], cheminformatics analysis revealed that the styryl compounds were less diverse: they possessed a narrower range of molecular weights (Figure 2A); radius of gyration (Figure 2B); logP (Figure 2C); fraction of rotatable bonds (Figure 2D) and number of hydrogen bond acceptors (Figure 2E).
This resulted in disappearance of the HC-band and appearance of bands of higher molecular weights (Figure 1B).
The AtCRT1b and CRT3 antibodies did, on the other hand, recognize bands with lower apparent molecular weights (Figure 3A).
Following treatment with MGO and proteasome inhibitors, HIF-1α that co-immunoprecipitated with CHIP migrated at higher molecular weights (Figure 6B), consistent with ubiquitinated forms of the protein.
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