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The red arrows indicate the CBP21s and Chi92s with molecular weight estimation of 20 25 or 91 96 kDa, respectively.
The E-PAGE MagicMark Unstained Protein Standard (Invitrogen, Carlsbad, California) was used for the molecular weight estimation of proteins after western blotting.
The resulting heme ladder was shown to be suitable as a protein standard for direct molecular weight estimation in immunoblot analysis due to the peroxidase activity of its constituents.
Furthermore, molecular weight estimation for SpGH38 by size exclusion chromatography indicated a molecular mass of 250 kDa (data not shown).
Molecular weight estimation of purified SAF showed the molecular weight to be ~71 kDa.
PageRuler™ Unstained Protein Ladder (Fermentas, Gothenburg, Sweden) was included for molecular weight estimation.
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Molecular weight estimations were made using an empirical mass vs. size calibration curve of the instrument software (Dispersion Technology Software 5.03, Malvern Instrument).
Molecular weight estimations of UP, which has an apparent molecular weight of 50 kDa in 16% SDS-PAGE gels and 72 kDa in 5 18% gradient gels, were also similar to UP [ 109].
SAXS measurements of unmodified full-length Parkin yielded an R g of 29.1 Å and a molecular weight estimations near its 52-kDa monomeric mass (Figs 2D and EV2C).
It is useful in molecular weight determination, relative estimation of purity, and amino acid sequencing of proteins.
Input parameters for estimation of molecular weight were considered as specific gravity and normal boiling point.
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