Scaffolds degraded over time, but no obvious differences in the rate of degradation were observed in low-molecular-weight constructs compared to high-molecular-weight constructs.
Based on obtained results, a soluble, and non-allergic protein with 40.59 kDa molecular weight was constructed.
This molecular mass is substantially larger than the predicted molecular weight of AnPGI constructs of 41.9 kDa (36.4 kDa of AnPGI plus 5.5 kDa of the added tags).
The molecular weights of construct 2 and 4 were estimated by MALDI-ToF to be 54.6 kDa and 58.5 kDa, respectively.
bCatalytic activity of Cel5A calculated under consideration of single enzyme's molecular weight in fusion construct.
A cDNA library of the Xcc low molecular weight RNA was constructed using the TaKaRa small RNA cloning kit DRR065 (TaKaRa, Dalian, China) and the experimental steps were performed according to the manufacturer's instructions.
The bound platinum moiety is present as an N,O Pt chelate at the distal end of a tetrapeptide linker, glycine-phenylalanine-leucine-glycine, and the weight-average molecular weight (Mw) of the construct is 22 kDa.
The additional molecular weight of 5.5 kDa for construct 2 and 7.3 kDa for construct 4 as well as the broad shape of the band in the SDS-PAGE suggest that both enzymes are glycosylated, a common phenomena for NA[12], [13], [15].
The molecular weights of these constructs measured on SDS-PAGE differ from the calculated molecular weights due to abnormal behavior of NF proteins on these gels [29].
The prominent bands appear at the appropriate molecular weights for our constructs.
For example, His-tagged Bgl1 accounts for 49.2% of total molecular weight (98.4 kDa) in fusion construct CB, indicating that vmax = 744.0 U per mg of total fusion protein can be adjusted to 1,256.7 U per mg of Bgl1 as fusion partner in CB.
