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Consistent with this observation and enhanced oxidative stress, older mice had significantly increased disulfide oxidized Atg7, at a molecular weight consistent with a disulfide-bound Atg3 heterodimer.
This loss in LC3 lipidation was due to increased Atg7 oxidation, which generated an oxidized complex at a molecular weight consistent with a disulfide-bound Atg3 heterodimer (Fig. 6d).
As expected, in the non-induced sample, a single band with apparent molecular weight consistent with the predicted molecular weight of Ty-PFC4 protein was observed.
Lysates from cells expressing FIT2 constructs with FNF insertions at positions 51, 129, and 217 that were treated with PNGase F decreased in molecular weight, consistent with glycosylation and the localization of these domains to the ER lumen.
Mutation of the GATA sequence shifted the DNA binding complex to a lower molecular weight, consistent with the absence of GATA-4 (Fig. 4B, lane 4 5 from the left).
Stripping and reblotting with an anti-GFP antibody confirmed that the most prevalent GFP containing species had a molecular weight consistent with the full length ROZA (Fig. 3A lower gel).
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Both GABARAPL1 and 2 formed complexes at molecular weights consistent with bound Atg3 and Atg7, which decreased following an hour of amino acid withdrawal and could be selectively cleaved using hydroxylamine.
C2-C4 concentratinns in Vial C fell over time proportional to their molecular weights consistent with solution in n-octadecane.
Molecular weight analysis indicated that many of the orange compounds that migrated more slowly during TLC separation had molecular weights consistent with them being SAB-R1 derivatives carrying an additional oxygen atom.
SDS-PAGE and Western blot analyses with anti-RGS-His and anti-GST antibodies revealed protein bands with apparent molecular weights consistent with the calculated molecular weights of the S. macrospora CAs (data not shown).
We have been able to detect cDNAs corresponding to both isoforms, and we have observed in western blotting two MBD5 bands, with molecular weights consistent with the predicted isoforms (see following section).
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