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Like other initiator caspases, caspase-2 activation requires dimerization that occurs in high molecular weight complexes.
The identity of the higher molecular weight complexes was further supported by co-immunoprecipitation studies.
Once again, we observed higher molecular weight complexes for Atg3 and Atg7 consistent with their stable covalent interaction with LC3 under non-reducing conditions (Fig. 3a), which were lost with beta-mercaptoethanol treatment.
However, on assessing Atg3 and Atg7 in HEK cells under non-reducing conditions, we observed higher molecular weight complexes (approximately an additional 15 kDa) consistent with covalent-bound LC3 (Fig. 2a).
BS69 contains regions of similarity to the PHD finger, the bromodomain, and the MYND domain, all of which are found in other proteins present in high molecular weight complexes that regulate transcription and/or modify chromatin structure.
Adiponectin is an insulin-sensitizing adipocytokine that circulates in plasma as multimeric isoforms, including trimers, hexamers and high molecular weight complexes.
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Herein we report that C4BP can form stable high-molecular weight complexes with soluble CD154 (sCD154), which despite binding CD40, completely inhibit CD40-mediated apoptosis of human cholangiocytes.
The coiled-coil region seems to be predominantly necessary for multimerization, resulting in the formation of high-molecular weight complexes.
Most of the USP7 and USP11 eluted in high-molecular weight complexes of between 400 and 700 kDa.
The latter could result in reduced solubility and formation of high-molecular weight complexes that would be expected to scatter light in the visible region.
Serum cystatin C-immunoglobulin high-molecular-weight complexes in kidney and liver transplant patients.
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