This impairment in autophagy correlated with an increase in inhibitory Atg7 oxidation in aged mouse aorta, which generated a molecular weight complex consistent with disulfide-bound Atg3.
In addition, we observed a higher molecular weight complex when only Atg7 and LC3 were present, which had a mass consistent with an oxidized Atg7 disulfide dimer.
Fisher, F. F. et al. Serum high molecular weight complex of adiponectin correlates better with glucose tolerance than total serum adiponectin in Indo-Asian males.
With a concomitant increase in oxidant generation, this could explain how oxLDL promotes formation of an oxidized higher molecular weight complex for Atg7 and prevents LC3 lipidation.
This observation was consistent with the ability of oxLDL to increase ROS, confirmed by elevated disulfide oxidation of Atg7, which generated a molecular weight complex corresponding to bound Atg3.
We find that both ATR and ATRIP associate with chromatin in vivo, and they exist as a large molecular weight complex that can bind single-stranded (ss DNA cellulose in vitro.
The requirements for both the expression and processing of PIDD for E1A-induced cytotoxic sensitivity (Figs. 2 and 3), indicate that the PIDDosome (a high molecular weight complex containing PIDD, caspase-2 and RAIDD) serves as the initiator complex for caspase-2 activation13.
In addition, there was a time-dependent accumulation of a high molecular weight complex of ligand and a cellular protein.
ExFlk.6His bound to endothelial cells only in the presence of VEGF, and cell surface cross-linking yielded a high molecular weight complex consistent with the VEGF-mediated formation of a heterodimer between ExFlk.6His and the endogenous VEGF receptor.
Exposure of the resultant high molecular weight complex to β-mercaptoethanol (β-ME) leads to dissociation.
We have recently identified an alternatively edited COXIII mRNA and have shown its protein product associates in a high molecular weight complex in trypanosome mitochondrial membranes [11].
