The mean-square radius of gyration and the intrinsic viscosity in toluene and cyclohexane determined as functions of molecular weight were analyzed with the aid of the theories for the wormlike chain model to determine the stiffness parameter λ−1 of the main chain.
We noticed that when cells were grown in both nutrient-rich and starvation conditions, the Atg31 protein displayed multiple bands of higher molecular weight when analyzed by SDS-PAGE (Fig. 1A).
The properties including aliphatic index (AI), GRAVY (grand average of hydropathy), extinction coefficients, isoelectric point (pI), and molecular weight were analyzed using this tool.
Mass spectrometry (MS) detection of high mass accuracy and resolution, such as provided by time-of-flight (TOF) instruments can yield accurate information on the molecular weight of analyzed proteins species.
The components of the whey in the hypoallergenic formulas (HF) were fractioned according to their molecular weight and analyzed by an enzyme-linked immunoaffinity chromatography method, showing that peptides of molecular weight less than 3,000 Da were antigenic and are probably allergenic (Puerta et al. 2006).
Separate experiments to characterize the formation of gradients inside the device in the absence of neutrophils were performed by replacing the fMLP with fluorescein (Sigma) of comparable molecular weight, and analyzing the distribution and changes in fluorescence intensity from time-lapse imaging.
Several standard proteins were analyzed under the same conditions to generate standard curves to estimate molecular weights of analyzed proteins.
Additionally, the pools of compounds excreted in feces attending to their molecular weights were analyzed.
A series of ethylene/1-hexene copolymers synthesized with a metallocene catalyst with varying comonomer contents but constant molecular weights were analyzed with crystallization analysis fractionation (Crystaf), solid state differential scanning calorimetry (solid state DSC) and solution differential scanning calorimetry (solution DSC).
Samples were then mixed with 2% SDS loading buffer of for 20 min. Their molecular weights were analyzed on a 3 12% Tris-Glycine gel in SDS running buffer.
