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Our protocol allows for individual molecular variation to be assessed.
Moreover, a hierarchical AMOVA did not attribute any of the existing molecular variation to differences between DLC-sensitive and DLC-tolerant groups.
Low nucleotide variation and extensive LD make investigation of selected targets difficult in C. elegans (Cutter 2006; Jovelin et al. 2009; Rockman and Kruglyak 2009; Rockman et al. 2010; Andersen et al. 2012), particularly if the aim is to tie molecular variation to a specific evolutionary context.
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In these cases, quantifying biological traits at single-cell resolution is invaluable because it offers the possibility to link molecular variations to the microscopic sources of phenotypic variation.
Still, these studies demonstrate the applicability of SRAP markers to unveil molecular variation correlated to a geographic scale.
Analyses of this molecular variation help to set priorities in conservation programs, to improve traceability in livestock, to estimate genetic diversity among breeds, etc. [ 16- 18].
Genes in the MHC in vertebrates are involved in pathogen resistance (Hedrick and Kim 2000; Bernatchez and Landry 2003), and molecular variation appears to be important in response to pathogens.
This type of molecular variation seems to be common in allogamous plants [86] and could also be a characteristic of autogamous species [33].
More specifically, since molecular variation contributes to cancer progression and survival then the present findings set the ground for targeting these mechanisms resulting in new therapeutic strategies of metastatic disease.
Molecular variation appears to be a better predictor of inbreeding effects in populations than variation in quantitative traits.
Few previous studies have employed such large collections of intraspecific sequence variants in order to analyze patterns of adaptive molecular variation at single genes [22].
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