To do this, we focused on the set of deletion-CNV regions in our molecular validation pipeline and examined each deletion's mean standardized log2 ratios.
Third, more translational research using human analogous animal models, where available, would make a significant contribution to the understanding of the disease and treatment by providing histological and molecular validation for both mechanistic and therapeutic drug studies.
RJL, SP, PK, and EAM carried out the molecular validation experiments in yeast, and JH and EJS performed the RNAi experiments in HeLa cells.
Cellular and molecular validation of this model was conducted in three phases.
With the growing popularity of RNA-seq for transcriptome studies, we expect PrimerSeq to help bridge the gap between high-throughput RNA-seq discovery of AS events and molecular validation of candidate events by RT-PCR.
One respondent suggested two weighting schemes, one which is purported for data containing microarray, clinical chemistry and histopathology measurements (suggestion 5a) and one for molecular validation of toxicological evaluations of the samples (suggestion 5b).
These deletions were grouped by strain, and then categorized as 'simple'complexlex' and 'false positive', depending on the molecular validation result of the corresponding CNV region (Figure 5A and B, and Figure S2 A to E).
We next assessed the false positive rates in aCGH by independent molecular validation of a subset of CNV regions (44 deletions and 17 gains).
Molecular validation experiments pinpointed the iron metabolism factors AFT1, CCC1, and YAP5 as contributors to these molecular and cellular phenotypes; in genome-scale sequence analyses, a suite of iron toxicity response genes showed evidence for rapid protein evolution in Malaysian yeast.
Comparisons between high-throughput genomic analyses of inducible GEM models of mammary cancer and human breast tumors will provide further molecular validation of specific models as representative of certain subtypes of human breast cancer [ 5, 7, 8].
