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To establish a reference sequence database for molecular typing, we cloned and sequenced the 5'NCR of all 101 HRV serotypes.
Utilizing two independent methods of molecular typing, we could show a large genomic diversity among the RT-associated S. aureus strains, which was especially remarkable since the patients were recruited from a comparatively small and stable population of about 350.000 inhabitants.
On the basis of phage typing and molecular typing, we identified 15 different MRSA strains isolated from two or more persons.
Using molecular typing we detected one C. pecorum genotype (denoted with ST 23), associated with these SBE cases from WA, as well as other SBE cases from Australia, England and the USA.
For molecular typing, we first used the species-specific oligonucleotide PCR assay Lib13, as described by Tanriverdi et al. (8 ), with a new sense oligonucleotide primer based on the genome sequences of C. hominis (9 ) and C. parvum (10 ).
For molecular typing, we used repetitive-element PCR with 18-mer degenerate primers REP1R-Dt (3′-CGGNCTACNGCNGCNIII-5′) and REP2-Dt (3′-CATCCGGNCTATTCNGCN-5′) (N = A, C, G, and T; I = inosine) (11 ).
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To determine the extent of recombination, if any, within each molecular type, we implemented three complementary tests: the ILD/PHT test, the Index of Association IAandand the phylogenetic incompatibility.
To determine the extent of clonality and recombination in populations of different molecular types, we used three different tests of linkage disequilibrium, (i) the incongruence length difference/partition homogeneity test (ILD/PHT), (ii) two measures of index of association (IA and rBarD), and (iii) the phylogenetic incompatibility test.
Using serotyping and molecular typing methods, we systematically investigated the association between isolates from liver aspirates and those from other body sites in patients with K. pneumoniae liver abscess.
On the basis of our molecular typing results, we strongly suspect that B. anthracis spores were accidentally introduced into the heroin supply in Turkey (or surrounding regions) before being smuggled into Europe.
Molecular typing Molecular typing Molecular typing was done using primers for amplifying and sequencing ompA, csuE and blaOXA-51-like gene fragments as described previously [ 24]. b.
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