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Molecular typing Molecular typing Molecular typing was done using primers for amplifying and sequencing ompA, csuE and blaOXA-51-like gene fragments as described previously [ 24]. b.
Molecular typing was performed on all MRSA isolated.
HLA molecular typing was performed by reference strand conformational analyses and/or sequence-based typing.
RAPD molecular typing was used to assess whether single or multiple strains of G. destructans were involved in the local occurrence of bat WNS.
Molecular typing was attempted on a representative subset of samples from each shedder, selected to cover all observed shedding episodes in this cohort (N = 116 samples).
Molecular typing was done with five random probes: a 15-bp minisatellite probe from M13 bacteriophage (5' GAGGGTGGCGGTTCT 3'); a microsatellite repeat (GACA 4 probe; and three 10-mer probes namely, OPA 1 (5'-AATCGGGCTG-3'), OPA 2 (5'-GTGATCGCAG-3') and OPA 3 (5'-GACCGCTTGT), from Eurofins MWG Operon (Huntsville, AL) [55].
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Culture and molecular typing were not performed as a clinical or infection control tool, therefore historical typing data were not available.
Knowledge of extant genetic lineages at particular times in past human populations can be obtained from well-preserved specimens where molecular typing is possible, using deletion analysis, microsatellite analysis and whole genome sequencing.
A flow chart of the sample collection, processing and molecular typing is provided in Fig. 2.
Blood sample collection and HLA molecular typing were carried out with national and local ethical consent.
Bacterial species detection, antimicrobial susceptibility testing and molecular typing were performed.
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