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By determining the nucleotide sequence of the amplicon, a molecular typing method was developed.
To determine the optimal molecular typing method, 60 P. aeruginosa isolates from sputum or throat swab cultures obtained from 58 different CF patients visiting the University Medical Centre Utrecht (UMCU) in 2007, were typed by PFGE, MLST and MLVA9-Utrecht.
RAPET is a rapid screening molecular typing method developed at the Mycobacterium Reference Unit (MRU) (3).
An ideal molecular typing method should accommodate the requirements with respect to both performance and analytical criteria.
Therefore, we used reliability of patient selection, molecular typing method, and statistical analysis method as quality variables.
However, in these studies, transmission of P. jirovecii could not be assessed at the molecular level because no molecular typing method for P. jirovecii existed.
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In addition to improving the method, comparisons between other molecular typing methods such as FAFLP or a sequence based typing scheme, would ultimately determine the validity of the MLVA assay as molecular epidemiology tool for L. interrogans.
We used molecular typing methods (pulsed-field gel electrophoresis [PFGE] and multilocus sequence typing [MLST]) to determine the relationships among B 2b with the parental C 2b P1.2,5 epidemic strain before and after the immunization campaign with the A+C polysaccharide vaccine.
Thus, molecular typing methods are required.
Our intention is to emphasize the recent molecular typing methods applied in genotyping Entamoeba species and strains, and to assess their value and limitations.
The present study was designed to evaluate the usefulness of two novel molecular typing methods, amplification of DNA fragments surrounding rare restriction sites (ADSRRS-fingerprinting) and the PCR melting profile (PCR MP), for Staphylococcus aureus strain differentiation.
More suggestions(15)
molecular clock method
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molecular diagnosis method
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molecular genotyping method
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molecular imaging method
molecular detection method
molecular biology method
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