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In eric-pcr, seven molecular types were observed with eric-Cl primer, and nine molecular types with eric-C2 primer.
When the results of both the eric-pcr were combined for molecular typing, 21 molecular types were observed, which indicated a high degree of discrimination.
Based on neighbor joining phylogenetic analysis [40], in which seven MLST loci were concatenated into 5.7 kb of contiguous sequence, the four discrete VG molecular types were clearly distinct, and the VGI group was separated into 20 sequence types (ST) (Figure 3B).
Possible coexistence of both ∼19 kDa and ∼18 kDa PrPres had already been suggested when variable proportions of these two PrPres molecular types were observed in the brains of TgOvPrP4 mice infected with two "CH1641-like" isolates (O100, O104) identified in V136R154Q171 homozygous sheep [6], [17].
Groups of cases infected with different P. jirovecii molecular types were compared to investigate differences in exposure histories.
The molecular types were recently confirmed by RFLP analysis of the orotidine monophosphate pyrophosphorylase (URA5) gene and the phospholipase (PLB1) gene (19 ).
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The number of laboratories contributing data for other molecular types was too low to ascertain that the differences were due to factors other than assay variation.
Within C. gattii, four molecular types are recognized (VGI to VGIV).
Genetic exchange between the four VG molecular types is rare, indicating that these likely represent cryptic species [10], [11].
Within C. gattii, four molecular types are recognized: VGI, VGII, VGIII and VGIV [22], which may in fact represent different varieties or phylogenetic species [23].
Culture and molecular typing were not performed as a clinical or infection control tool, therefore historical typing data were not available.
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