This paper summarizes the longterm outcome, according to molecular type, of 166 consecutive SCID infants given non-conditioned related donor bone marrow transplants at this institution over the past 28.3 years and reviews published reports of longterm outcomes of transplants in SCID performed at other centers.
URA5-RFLP analysis using the enzymes HhaI and Sau96I was performed initially to verify the molecular type of each studied strain as previously described [9].
The environmental investigation found that one of the soap dispenser, where HCWs used to wash their hands, was heavily contaminated with the same molecular type of P. aeruginosa as confirmed cases which is compatible with an environmental common continuous source of infection as above hypothesized.
Seventy three strains composed of at least ten representative strains of each haploid molecular type of the Cryptococcus species complex from different parts of the world, and the outgroup species Cryptococcus albidus were retrieved from the Molecular Mycology Research Laboratory culture collection at Westmead Hospital, University of Sydney, Westmead, NSW, Australia (Table 1).
However, the emergence of cryptococcosis due to an uncommon molecular type of C. gattii (VGII) in humans and animals on Vancouver Island, BC, Canada, and its more recent spread to mainland British Columbia and the adjacent Pacific Northwest of the United States has changed this paradigm [4], [5], [6], [7], [8], [9].
The unique molecular type of both Y. pseudotuberculosis isolates and the atypical clinical course of infection will be comparatively discussed.
Molecular typing of HLA-E.
Since the mid-1990s, PFGE has been the mainstay for molecular typing of NTM.
Trovato, A. et al. Molecular typing of Mycobacterium abscessus isolated from cystic fibrosis patients.
van Ingen, J. & van Soolingen, D. Molecular typing of nontuberculous mycobacteria.
Santin, M. et al. Incidence and molecular typing of Mycobacterium kansasii in a defined geographical area in Catalonia, Spain.
