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Doctors could perform a small biopsy with a needle, check for the molecular type, and give the right breast cancer tablets.
Based on phenotypic and molecular methods, both isolates were C. gattii, VGI molecular type, and distinct from the Vancouver Island outbreak isolates.
However, three strains of each species revealed some incongruence between the original molecular type and the sequence-based type obtained here.
The molecular type and mating type of each strain were confirmed by URA5 gene RFLP analysis [12] and mating type specific PCR [8].
Since clonal reproduction can mask the effect of recombination, we prepared two different databases: one included all strains of each molecular type and the other included the clone corrected data of each molecular type from which identical genotypes were removed.
A correlation has also been reported between molecular type and virulence gene expression [ 28, 29].
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The infections were predominantly with Enterobacter cloacae, and molecular typing and serotyping showed these isolates to be indistinguishable.
Here we present a simple and efficient method based on a multilocus SSR analysis for molecular typing and genetic diversity assessment of L. thermotolerans isolates.
For both species the obtained phylogenetic trees correspond to these molecular types and are highly supported by both maximum parsimony and Bayesian analyses.
However, we believe that CSSA represents a step forward in prion molecular typing and offers several advantages compared with previously used protocols.
The molecular typing and characterization of the population biology of SDSE in this report provides a foundation for future studies that address the evolution and molecular basis for virulence in SDSE.
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