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In this review we describe the expanding repertoire of molecular tools with which to study gene function in Leishmania.
We have developed monoclonal antibodies directed against the pseudopeptide ψ-130, derived from the highly conserved malarial antigen Plasmodium falciparum merozoite surface protein 2 (MSP-2), for obtaining novel molecular tools with potential applications in the control of malaria.
Unraveling signaling pathways in primary neurons has been a central issue for neurobiologists and pharmacologists, but the analysis has been hampered by the paucity of specific molecular tools with which to dissect signal transduction cascades.
are impressive demonstrations of the power of melding new molecular tools with more classical epidemiologic studies.
Meanwhile, identification of M. africanum requires confirmation by molecular tools with a better discriminatory power than spoligotyping.
This explains the reluctance on the part of researchers to use the RAPD method and their search for newer molecular tools with a better diagnostic performance.
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Molecular tools compatible with the analysis of very small amounts of material have resulted in research aimed at designing molecular criteria to define this competence.
These molecular tools together with the ability to record and label individual MSNs in their entirety in vivo and examine the same neurons in the electron microscope enabled us to address directly the innervation of direct and indirect pathway MSNs by the cortex and thalamus.
The application of different molecular tools, in combination with other techniques to the problem will be illustrated.
In some cases, polymorphisms that disrupt 14-3-3 14-3-3 14-3-3have been linteractionsausative way to disease, and identifying furthavecases therefore has implications for the discovery of new disease beenarkers and for the develinkedt of molecular tools that interfere with 14-3-3-phosphoprotein interacausative4, 35).
Moreover, a better understanding of the pathways (such as the Wnt/β-catenin signaling pathway) that trigger EMT and cancer cell self renewal (cancer stem cells) might lead to new therapeutic approaches for cancer patients by developing molecular tools that interfere with them.
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