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The newly isolated bacterial strain was identified by molecular techniques using 16S rDNA sequencing.
To aid conservation efforts, molecular techniques using polymerase chain reaction (PCR) have been developed that can identify whether marketed shark parts (e.g. fins) are from prohibited species like the dusky shark, versus similar allowed species such as the sandbar shark.
Research has been conducted to develop molecular techniques using different types of samples to diagnose this parasitic disease [ 31- 33].
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Advances in vaccine technology are occurring in the molecular techniques used to develop vaccines and in the assessment of vaccine efficacy, allowing more complete characterization of vaccine-induced immunity correlating to protection.
Standard molecular techniques used to make constructs were as described in Sambrook and Russell [ 48].
Molecular techniques used for definitive diagnosis, specifically PCR, are available mostly in public health laboratories and research settings.
The last two articles in the series deal with molecular techniques used to study pre-invasive breast lesions.
Among the molecular techniques used in clinical and field diagnosis, (RT -PCR is consideRT -PCReference standard because of iss versatility and rapid turnover.
The section provides the reader with the fundamentals of the present-day molecular techniques used to acquire and analyze multi-gene expression and specific gene sequences.
Growth, handling of bacteria and yeast, and all the molecular techniques used in the study were according to standard protocols (Sambrook et al. 1989).
How can we find solutions if the molecular techniques used show errors and many times we are unable to reproduce the same findings in different labs.
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