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DGGE analysis followed molecular techniques described elsewhere [44, 45].
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29 31 In contrast, recent work employing sensitive molecular techniques describes two healthy infants shedding a diverse range of enteric viruses almost continuously throughout the first 12 14 months of life.
Construction and expression of the recombinant N-terminal glutathione S-transferase (GST -tagged protein was performed usinGST -taggedmolecular biology techniques described proteinsly.
The molecular and electrophysiological techniques described here in combination with behavioral assays will facilitate identification of key stimuli and those protein or effector components of the Anopheles olfactory system responsible for their recognition.
Virus infection of test plants was judged by the appearance of characteristic symptoms caused by each of the two viruses [39], [40] and further confirmed by molecular techniques as described below.
Standard molecular techniques are described by Sambrook et al. (1989).
Microbial and molecular techniques were described previously (Burke et al. 2000).
Microsatellite instability (MSI) status for tumor samples was determined using molecular techniques as described by Woods et al. [ 8].
The sex of each animal collected was assessed both during field manipulations and using molecular techniques as described in Shaw et al. (2003) (Table 1).
After this screening procedure, carbapenemase producers may be identified according to the techniques described above (antibacterial drug susceptibility testing, molecular techniques).
A total of nine specimens was found, examined and afterwards fixed for the techniques described below as well as for molecular analysis.
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