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Fukui indices to determine the local reactive site for the molecular systems during electrophilic, nucleophilic, radical and dual descriptor attacks.
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We included and compared all pVLs of patients who had ≥1 pVL assessed via RT-PCR (Roche AMPLICOR HIV-1 MONITOR Ultrasensitive version 1.5, Roche Molecular Systems, Inc) during eight months prior to assay change, and via bDNA (VERSANT HIV-1 RNA version 3.0 bDNA Assay, Siemens Diagnostics) during six months after the change.
First-strand cDNA was generated with 2.5 μM oligo d(T 16, 5 mM MgCl2, 1 mM dNTPs and 1 U of RNase inhibitor (Applied BioSystems, Roche Molecular Systems, Inc., Branchburh, NJ, USA) during 1-h incubation at 42 °C.
It is crucial for cellular/organismal homeostasis during lifespan that molecular systems can adapt and retain functionality despite long-term variation of environment.
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