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Effectiveness was further improved by incorporating the GDEPT strategy into a novel molecular switch system using the Cre/loxP recombinase system of bacteriophage P1.
As stated in the introduction, there are certain criteria for a molecular switch system.
According to Toniatti and co-workers, there are several criteria for an effective molecular switch system.
This work focuses on characterizing a molecular switch system that quantitatively controls transgene expression.
The molecular switch system was designed to contain an engineered NR, a promoter region, and a target transgene.
One way to introduce stable expression of the molecular switch system is to infect cells using a retrovirus.
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The concerns posed by these systems permit the development of new or improved molecular switch systems.
One of the most widely used methods to control gene expression is molecular switch systems.
As discussed previously, three successful ligand-dependent molecular switch systems have been used for various cellular studies; however, these systems contain certain limitations for in vivo applications.
These ligand/receptor pairs are ideal for molecular switch systems because of their selectivity and lack of interaction in other cellular pathways.
Molecular switch systems that activate gene expression by a small molecule are effective technologies that are widely used in applied biological research.
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