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The details on RNA extraction, RT-PCR, and PAM50 Breast Cancer Intrinsic Classifier™ (PAM50, ARUP Laboratories, Salt Lake City, Utah) molecular subtyping assignment were according to previously published methods [ 31].
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Molecular subtype assignment according to the subtype clustering method [ 17].
We compared gene expression and molecular subtype assignment of matched tumors collected at different time points.
Finally, our results regarding changes in molecular subtype assignment must be interpreted with caution, owing to the small sample size and the lack of consensus for molecular subtype assignment.
We analyzed a series of 2,658 ERα-positive HER2-negative breast tumors for ERα and progesterone receptor (PR) status as determined by mRNA expression and for their molecular subtypes (Luminal type vs Basal type, assessed by BluePrint™ molecular subtyping assay).
Next, we integrated EOC cell line data into the molecular subtyping scheme to derive an in vitro working model representative of each molecular subtype.
The PulseNet surveillance system is a molecular subtyping network of public health and food regulatory agency laboratories designed to identify and facilitate investigation of foodborne illness outbreaks.
The most common molecular subtyping method is PFGE.
Molecular subtyping analysis using pulsed-field gel electrophoresis showed 2 major clusters.
Discriminative molecular subtyping methods are necessary for timely outbreak detection and tracing of contamination sources.
Molecular subtyping of breast cancer may provide additional prognostic information regarding patient outcome.
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