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Of the 20 FFPE and FF pairs, 19 showed an agreement in terms of molecular subtype prediction across all replicates.
Thus, the molecular subtype prediction model can assign clinical samples with unknown subtype status with high accuracy.
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The different classes of molecular subtypes predict patient prognosis 22, 23 and aid in prediction of response to neoadjuvant treatment in node-negative patients 25.
For comparisons of molecular subtype classifications, we applied a commonly used classifier designed for single sample predictions, the 50-gene subtype classifier (PAM50) developed by Parker et al. [ 18].
Using prediction analysis for microarrays [ 27], predictors for breast cancer receptor status (ER, PR, HER2) and molecular subtype (Luminal A, Luminal B, Her2, Basal) were implemented using samples and clinical data (was not available for every sample) provided by TCGA.
Bertucci, F., Finetti, P. & Birnbaum, D. Basal breast cancer: a complex and deadly molecular subtype.
Masuda, H. et al. Comparison of molecular subtype distribution in triple-negative inflammatory and non-inflammatory breast cancers.
Molecular characterization and novel therapeutic targets of each molecular subtype have been identified.
The biological conformity observed within each consensus molecular subtype (CMS) holds promise for the design of subtype-specific treatment regimens.
Molecular subtype was determined on the basis of genomic analysis.
Our findings did not support an association between the uptake of either radiopharmaceutical and molecular subtype.
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