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However, the results do not establish whether similar molecular steps are affected in both organisms.
The benefits of this protocol include that it is straightforward to implement, and that the molecular steps are scalable to large numbers of individuals.
Although conidial germination is a crucial step in the life cycle of all filamentous fungi, it is surprising that the basic molecular steps are poorly understood [ 1].
Molecular steps are required for autophagosomal formation that involves a double-layered vesicle that fuses with a lysosome to digest the material inside the vacuole [ 17].
The molecular steps are scalable to large numbers (hundreds) of individuals and all steps post-DNA extraction can be carried out in 96-well plate format.
The molecular steps are scalable to large numbers (hundreds) of individuals and all steps post-DNA extraction can be carried out in 96-well plate format (throughput is often a limitation in other protocols).
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The percentage decay curves that showed clear molecular steps were typically 8 15% and were all selected for building the histograms.
Our results, both from experiment and simulation, suggest that the rate-limiting molecular step is formation of extended trimers.
Thirdly, the adjacency list file and the molecular features in the above two steps are provided to calculate the final interaction features.
Hence despite a common mechanistic role, the precise molecular interactions underpinning distinct intracellular transport steps are potentially divergent.
Despite having a well-characterized morphological basis for the precancerous cascade, studies showing the underlying molecular mechanism of progression through its steps are sparse.
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