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Exact(6)
The half DVD-Ig molecule showed molecular size at approximately 100 kDa and 95% monomer analyzed by size exclusion chromatography (Fig. 2B).
However, a non-specific band with molecular size at about 50 kDa was detected by anti-CRY1Ab polyclonantibodiesins in both MD44 and transgenic J. curcas plants.
However, the hybridization band had molecular size at about 20 kb, much greater than the expected 6565-bp PmlI- PmeI fragment (Fig. 4f).
These GlyAspGlyGly repeats would introduce a region of small glycine residues punctuated by charged aspartates causing a difference in molecular size at the N-terminus of the protein.
C2A with molecular size at about 30 kDa was detected and its expression level did not show significant difference in young leaves between MD44 and the transgenic T0 plants (Fig. 2b).
The initial results indicated that only one T0 plant, L10-T0, had Cry1Ab/1Ac expression with the expected molecular size at about 68 kDa; the remaining T0 plants either did not show any expression of the Cry1Ab/1Ac protein or expressed truncated Cry1Ab/1Ac proteins (data not shown).
Similar(54)
Finally, in rat primary glial cells, TIAF1 is expressed as 17- and 24-kDa proteins and high molecular sizes at a high cell density (Supplementary Figure S9).
Some of the results from this analysis should be considered an artifact of the contents of PubChem in that the population as a function of molecular size peaks at 26 non-hydrogen atoms and then rapidly declines.
The IPG strip was then transferred on a pre-casted 12.5% SDS-PAGE gel (GE Healthcare) and second dimensional electrophoresis, separating proteins in function of their molecular size, was run at 22°C for 1 h at 2.5 W/gel followed by 5 h at 17 W/gel on an Ettan DALT six vertical electrophoresis system (GE Healthcare).
After screening multiple additives in water solution in order to model the behavior of ions in concrete, the team found that those with a smaller molecular size were more successful at slowing the rate of ion diffusion.
If the lack of binding exhibited by the tetramer is due to size effects, this would set the upper limit of molecular size for molecular imprinting at approximately 20 30 Å.
Related(18)
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