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The difference of the half-life of the drug-target complex between vorinostat and MS-275 indicates that the lifetime of the drug-target complex is at least partially determined by the molecular set up of the inhibitory agent [ 64].
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With this molecular set-up the penetration through cell membranes is much better and the accessibility of the peptides to receptors enhanced.
Enzyme activity was monitored with a spectrophotometer (VersaMax™ microplate reader, Molecular Devices, CA) set up at a wavelength of 420 nm (i.e., OD420) for the ABTS substrate.
An innovative combination of various recently described molecular methods was set up to efficiently identify regions flanking a marker DNA in insertional mutants of Chlamydomonas.
Initial models optimized by molecular mechanics were set up in order to minimize the energy of an isolated chain and then located in the crystalline lattice by using the unit cell parameters obtained from the X-ray diffraction measurements.
A molecular test was set up with the L3 segment as target with primers enabling detection of the 3 reovirus serotypes (Table 1).
A rational molecular engineering strategy was set up to prepare an original series of efficient carbazole-based sensitizers for dye-sensitized solar cells.
The molecular diagnostic laboratory was set up in Mercy Hospital Research Laboratory (MHRL) located in the city of Bo, Sierra Leone.
To define the molecular mechanism involved, we set up in vitro EGFR Cbl association assays and EGFR ubiquitination assays.
In this sense, reverse dot-blot (RDB) hybridization using membrane-based macroarrays represents a cost-effective alternative that needs less specialized equipment and can be used in any well set up molecular biology laboratory [ 8].
After gaining a Wellcome senior fellowship, in 1987 he moved on to UCL, where he set up a molecular psychiatry laboratory.
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