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To delimit species using molecular sequences, we initially need to define our species concept.
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Since there is a growing need for the Alliaceae and Apiaceae research community to have direct access to family-specific molecular taxonomy sequences, we have parsed and extracted phylogenetic markers (e.g., ITS1 & ITS2, Non Transcribed Spacer (NTS), External Transcribed Spacer (ETS)) and "barcoding" genes (e.g., matK) associated with Apiaceae and Alliaceae families from GenBank sequence data.
To test this hypothesis and directly place α-SNAP in the molecular sequence of SOCE, we first asked whether the final molecular step of CAD domain mediated activation of SOCE via Orai1 is intact in α-SNAP depleted cells.
Based on a large panel of the entire N- and GPC-encoding DOBV sequences, we report direct molecular evidence that DOBV in Germany is represented by a genetic lineage associated with A. agrarius (DOBV-Aa).
To determine the relative performance of BI and ML when larger phylogenetic problems and real molecular sequence data are analyzed, we examined a well-known case of phylogenetic error (Fig. 3a).
We compared molecular sequence data from 3 genes and a variable region of the poxvirus genome (Table 1) among BRZ-VACV isolates, available vaccine strains related to those used during the eradication campaign in Brazil, and other VACVs isolated from domestic animals (including endemic buffalopox virus in India [ 1517 17 ] and horsepox virus [HSPV] from Asia [ 18 ]).
Here we present a molecular sequence character-based key for diagnosing Thunnus species, and compare its performance with phenetic approaches for identifying market samples of tuna.
Using several global approaches, which include a combination of biocomputational methods, molecular biology, and high throughput sequencing, we are identifying and characterizing small non-coding RNAs of P. syringae.
Molecular sequencing enabled identification of P. micra.
With new molecular tools such as PCR and sequencing, we can now identify much more precisely the rickettsial agent responsible for the disease.
Given the availability of considerable nuclear transcriptome sequence, we also assayed molecular evolutionary rates across a random set of 100 genes homologous to Conserved Orthologous Loci (COS II) available for several other asterid species [ 73- 75].
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