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Our detailed transcriptome-wide and functional analyses of these mice enabled us to identify the molecular role of PTCD1 as a 16S rRNA-binding protein essential for its stability, pseudouridylation, and correct biogenesis of the mitochondrial large ribosomal subunit.
The core autophagy machinery comprising 18 autophagy-related (Atg) proteins in yeast plays an essential role in autophagosome formation; however, the molecular role of each Atg factor and the mechanism of autophagosome formation remain elusive.
We used fluorescence microscopy and molecular genetics to examine the molecular role of MinJ.
To further understand the molecular role of Tyr176 in cell growth, we generated a HA-tagged myristoylated Y176F or myr-Y176F (Fig. 4C).
While, mutagenesis of K149 showed that it had a catalytic role to play during PLP biosynthesis [22], the exact molecular role of this lysine residue has been a matter of controversy in the literature.
In particular, our data provide the first in vivo evidence that the HVR of an M protein plays a major role in virulence, focusing interest on the molecular role of this region.
Rather this functional conservation probably reflects conservation of a cellular molecular role of HCF-1 proteins that is co-opted by the virus for the purposes of regulating infection.
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A better understanding of the molecular roles of immune complexes containing receptors, OsRac1, and chaperones is becoming increasingly important for the study of innate immunity in plants.
Given the diverse functions of ubiquitination, similar studies with ubiquitin attachment site mutants are becoming increasingly important in understanding the molecular roles of ubiquitination.
However, the precise molecular roles of Polδ and Polε remain unclear.
Therefore, careful evaluation of the redox state and of the substrate availability will be of further need to decipher the molecular roles of enzymatic RBPs.
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