The structures were determined by molecular replacement using Phaser and using Pvs25 as a search model46.
Structures were solved by molecular replacement using PHASER and refined with phenix.refine in the PHENIX suite66.
Phaser45 was used for molecular replacement using Fabs from our internal database as starting models.
The structure was determined via molecular replacement using the coordinates for VBC (PDB code: 1lm8 38.
The PBP2 structure was solved by molecular replacement using the structure of PBP3 from P. aeruginosa 15 as a model and employing BALBES35.
Eventually we solved the structure at 3.6-Å resolution by molecular replacement, using the K-8794-bound K-8794-bound K-8794-boundd Fig. 1a).
The bosentan-bound structure was determined by molecular replacement, using the K-8794-bound K-8794-bound K-8794-boundly rebuilt and refined astructureed andve.
We solved the structure at 2.2-Å resolution by molecular replacement, using the ligand-free structure of ETB-Y5-mT4L (PDB 5GLI) (Table 2 and Fig. 1a).
The structures of TyrBm with bound inhibitors were solved by molecular replacement using Phaser53 and the coordinates of earlier determined TyrBm structure (PDB code 4P6R).
The structure was determined by molecular replacement using the program PHASER [20].
The structure was solved by molecular replacement using the program AMoRe [39].
