Sentence examples for molecular replacement technique from inspiring English sources

The structure of the EGFP variant was solved by the molecular replacement technique using 1EMG [20] as a model.

Mutant structures were determined with the Molecular Replacement technique using the program CNS [34] and the wt-KaiC structure with PDB ID 3DVL [19] as the search model.

The structures are homologous to that of wt-KaiC and diffraction data were phased using the molecular replacement technique and the KaiC structure with PDB ID code 3DVL [19] [19].

The crystal structure of nonfucosylated Fc complexed with the bis-glycosylated sFcγRIIIa was determined by a molecular replacement technique with the crystal structure of a human fucosylated IgG1-Fc fragment complexed with nonglycosylated FcγRIIIb (PDB ID code 1E4K) (Sondermann et al. 2000) and refined to 2.2-Å resolution (Table 1).

To build the atomic model of the triple mutant (1FBK), amplitude data derived directly from experimental diffraction patterns of the mutant was used [27] in a strategy similar to that used for molecular replacement techniques used in X-ray crystallography.

The absence of experimental phase information is a common scenario in X-ray and electron crystallography when molecular replacement techniques are used to determine structures of proteins using only diffraction data in combination with the previously known structure of a closely related protein.

We show how this application can be used to produce standardized structural comparisons, identify common substructures among structurally related proteins, as well as generate sieved models for use in protein crystallographic structure determination using the technique of Molecular Replacement.

The application addresses two fundamental problems in structural biology: First, the identification of common substructures among structurally related proteins an important problem in characterization and prediction of function; second, generation of sieved models with demonstrated uses in protein crystallographic structure determination using the technique of Molecular Replacement.

Molecular replacement (MR) is a popular protein crystallographic technique that exploits the structural similarity between proteins that share some sequence similarity.

Although there are other methods of structure determination, molecular replacement is predicted to become an increasingly common technique, for two reasons.

The structure for protein DX was initially solved by molecular replacement using the coordinates from protein 18-19, and later solved independently using single anomalous dispersion techniques.