Initial phasing was performed by molecular replacement using the software package CNS 1.2 and the structure of the binary complex of BMP-2 bound to BMPR-IA [PDB 1REW] as a search template; full technical details of the initial phasing analysis have been published elsewhere [ 67].
The structure was solved by molecular replacement using Phaser software [22] starting from a tetrameric model built by the SWISS-PDB server using as a template the crystal structure of PuuE allantoinase (PDB ID code 3CL6 [11]).
The structure was solved by molecular replacement using Phaser software [34] and the equine P2 structure (PDB entry 1YIV, [12]); the crystal form had one monomer per asymmetric unit.
The structure was then solved by molecular replacement using the software Phaser and the structures of BMPR-IAEC (from the complex BMP-2 BMPR-IA, PDBMP-2 BMPR-IA and of the FaBMP-2 BMPR-IAffinity for the tetanus toxoid (PDB entry 1REW).
The structures were solved by molecular replacement with the PHENIX software suite using an initial search model composed of the Tth 70S ribosome (PDB ID codes 2WDG, 2WDH, 2WDI, and 2WDJ) with all ligands and ions removed.
The structure was solved via molecular replacement using the PHASER crystallographic software with Ac-AChBP/GIC (PDB code 5CO5) as search model (McCoy et al., 2007).
The structures of PTPN18-HER2-pY, pY and pY were determined by molecular replacement with Phaser in the CCP4 software package.
There have also been key developments in software, and in molecular replacement and phase extension methods designed to facilitate the process of structure determination.
The structure was solved by molecular replacement (MR) as implemented in the software PHASER (McCoy et al., 2007 ).
