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The molecular recombination map further indicates that the IDLE MITE transposons are distributed throughout the genome and are relatively stable.
The existing molecular recombination map for Antirrhinum was built using the F2 of a cross between A. majus (line 165E) and a wild relative, A. molle [ 14].
The molecular recombination map currently available for the model eudicot Antirrhinum majus is the result of a cross with Antirrhinum molle, limiting its usefulness within A. majus.
To construct the molecular recombination map for the F2 population, co-dominant markers were scored as one of three allelic states (homozygous 165E, homozygous Sippe 50 or heterozygous) while dominant markers were assigned to one or other parent and scored for the presence or absence in F2 individuals.
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The alternative approach of gene isolation by positional cloning is currently restricted by the availability of molecular recombination maps in Antirrhinum, though it has recently been successful in isolating the fistulata (fis) gene [ 13].
These results indicate that the VB method enables the minimal use of molecular markers and efficient screening via an accurate recombination map.
Needing an accurate genome sequence for its gene-hunting program, Decode created a so-called recombination map of the human genome.
The August 2001 version, the latest analyzed, was a "real improvement," the Decode scientists say, but still contained 104 large-scale errors, according to their recombination map.
The SNPs are then examined by the script, Seq2Bin, for genotype calling, recombination breakpoint determination, and recombination map construction.
A whole-genome amplification method with reduced bias yields a personal meiotic recombination map.
For the recombination map, we assumed a flat recombination rate of φ = 10−8 per base pair.
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