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Images of the original Western blots used in this study are available in the Supplementary data; antibody detection characteristics across the full molecular range are available for membranes which were not pre-cut prior to immunodetection.
Hep (Sigma-Aldrich) had a molecular weight range from 6 30 kDa; fluorescent FITC (5-fluorescein isothiocyanate -Hep (λex = 491 nm, λem = 516 nm; Polyscisothiocyanate -Hepular range from 17–19 kDa.
Its amino-terminal fragment (N-CTGF), the predominant form of CTGF detected in plasma, has a molecular weight in the middle molecular range (18 kDa).
At this time, most clinicians appear to prefer hemofiltration or hemodiafiltration in critically ill patients with AKI, because of the belief that convective clearance might benefit patients by better removal of toxic inflammatory solutes, which are in the middle molecular range.
For control, lysates of Drosophila melanogaster were run in parallel, revealing a reactive band in the same molecular range (Fig. 1A, inset).
Interestingly, while TAF4, TAF5 and TAF6 eluted from the column as a single peak in the 1 2 MDa range, TAF8 and TAF10 were detected in at least two peaks eluting from the column in a molecular range of 1 2 MDa and about 400 500 kDa.
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In Figures 7 8, we show two individual mass spectroscopy scans that focus on molecular ranges around 270.
The authors find that a hexa-peptide of the PKA kinase, a known regulator of RyR2, has a very high affinity for RyR2, in the nano-molecular range, and that its binding site is connected to two known disease-associated residues (A77 and R176) through a "path" of residues predicted to be "energy responsive" through ENM calculations.
Spectra for each individual were collected over a low molecular weight range (2000 30 000) and high molecular weight range (20 000–150 000) using fixed (optimised) laser intensity and detector sensitivity settings.
The molecular weights range from 32 to 2.7 kDa.
More than 300 proteins were detected and localised between pI 4 7 and molecular mass range of 20 200 kDa.
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