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Our aim was to apply molecular protocols such 16S rRNA PCR, DNA sequencing and MLST analysis to an archival blood sample from a complex clinical case, which was reported as culture negative for bacterial growth after original presentation.
It may be useful to incorporate the routine use of molecular protocols such as 16S rRNA PCR and sequencing as an initial screen for microbial pathogens in clinical samples, this would allow more selective and efficient use of traditional culturing for confirmation, the cost of the molecular methods being offset by using less traditional culture associated consumables.
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Furthermore, RNases are ubiquitously expressed in tissues and RNase products are commonly used in molecular biology protocols (such as plasmid DNA preparation).
Traditional bacterial culturing techniques have many deficiencies, necessitating a search for adjunctive or alternative approaches such as molecular protocols.
Human soldiers have legal protocols such as the Geneva conventions to guide them.
Half-truths and fanciful speculation, shorn of academic protocols such as footnotes, can sound donnishly authoritative.
Reliable transport protocols such as TCP (Transmission Control Protocol) and SCTP Stream Control Transmission Protocoll) are designed to tolerate reneging.
Commonly used protocols such as the Transmission Control Protocol (TCP) and User Datagram Protocol (UDP) are also discussed.
It introduces to routed protocols, such as IP and discusses how protocols work.
SIP is also an application-layer protocol and works together with other application-level protocols such as RTP (real-time transport protocol) or SDP (session description protocol).
Specifically, quantum cloning machines can be designed to analyze the security of quantum key distribution protocols such as BB84 protocol, six-state protocol, B92 protocol and their generalizations.
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CEO of Professional Science Editing for Scientists @ prosciediting.com