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Other molecular protocols including cDNA or cRNA labelling, hybridization, PCR and sequencing are described elsewhere [ 33, 45, 46].
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High molecular weight DNA was prepared from frozen tumour material using standard protocols including proteinase K treatment, chloroform treatment and ethanol precipitation.
Protocols include unicast and multicast, Transact Protocol (TCP), User Datagram Protocol (UDP), and Real-Time Protocol (RTP).
Treatment protocols include chemotherapies and brain irradiation.
Both protocols include a DNase step.
Furthermore, not all protocols include extubation criteria.
The siderophore producing bacterium (S33) was subjected to a detailed identification protocol including morphological, biochemical and molecular identification.
In order for a molecular signature to be fully understood by other researchers, detailed information on the experimental protocol, including the patient selection criteria and experimental and analytic procedures, must be made available.
For a more detailed protocol including buffers, see Millipore's online ChIP protocol.
The analysis protocol includes molecular beam sampling, sorting the charged particles constituent according to mass/charge ratio (m/ze) via electrostatic deflection, and detection of the neutral particles by monitoring the variation of the quartz crystal oscillation frequency upon exposure to the particle – laden molecular beam.
For the evaluation of the Amplicor® Cystic Fibrosis kit (Roche Molecular Systems, Alameda, CA, USA), the extraction protocol included in the kit was tested, as well.
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